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Table 3 Comparative analysis of in-house screening ELISA assay, commercially available ELISA assay and HCV RNA PCR as reference standard

From: Expression of core antigen of HCV genotype 3a and its evaluation as screening agent for HCV infection in Pakistan

 

Core antigen assay

Commercial assay

PCR

'Gold Standard'

HCV true positive

36 (100%)

35 (97.2%)

36

HCV true negative

83 (98.8%)

80 (95.2%)

84

HCV false positive

0 (0%)

1 (2.88%)

0

HCV false negative

1 (1.2%)

4 (4.76%)

0

Total

120

120

120

  1. Sensitivity of our assay and commercial assay was 100 and 97.2%, while specificity was 98.8 and 95.2% respectively. The HCV RNA PCR was used as a standard reference in the comparison.
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Virology Journal

ISSN: 1743-422X