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Table 2 Viral infection in the blood sucked female mosquitoes by using the RT-LAMP unit, real-time RT-PCR, and one-step RT-PCR

From: Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay

Positive pools/pools of mosquitoes examined (viruses)

 

Culex tritaeniorhynchus,

Aedes aegypti,

Aedes albopictus,

RT-LAMP unit

18/135 (JEV)

3/98(DENV1, 3)

1/46 (DENV1)

Real-time RT-PCR

18/135 (JEV)

3/98 (DENV1, 3)

1/46 (DENV1)

One-step RT-PCR

10/135 (JEV)

1/98 (DENV1)

0/46

  1. DENV, dengue virus; JEV, Japanese encephalitis virus; RT-PCR, reverse-transcription polymerase chain reaction; RT-LAMP, reverse-transcription loop-mediated isothermal amplification.
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Virology Journal

ISSN: 1743-422X