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Table 1 Identification of DENV 1-4 and JEV by the RT-LAMP unit, real-time RT-PCR, and one-step RT-PCR using viral templates at 105-fold dilution

From: Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay

Viruses No. of strains No. of strains correctly identified with primer sets of DENV 1-4 and JEV, respectively
   One step RT-PCR Real-time RT-PCR RT-LAMP unit Cross-reaction of RT-LAMP *
DENV-1 17 15 17 17 0
DENV-2 13 11 13 13 0
DENV-3 10 9 10 10 0
DENV-4 12 11 12 12 0
JEV 14 12 14 14 0
Influenza viruses** 12 0 0 0 nd
Hantaviruses** 12 0 0 0 nd
  1. *A given primer set in the RT-LAMP unit was used to amplify other viral templates. ** These viruses were amplified with the primer sets of the five flaviviruses, individually. nd, not done. DENV, dengue virus; JEV, Japanese encephalitis virus; RT-PCR, reverse-transcription polymerase chain reaction; RT-LAMP, reverse-transcription loop-mediated isothermal amplification.