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Table 1 Identification of DENV 1-4 and JEV by the RT-LAMP unit, real-time RT-PCR, and one-step RT-PCR using viral templates at 105-fold dilution

From: Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay

Viruses

No. of strains

No. of strains correctly identified with primer sets of DENV 1-4 and JEV, respectively

  

One step RT-PCR

Real-time RT-PCR

RT-LAMP unit

Cross-reaction of RT-LAMP *

DENV-1

17

15

17

17

0

DENV-2

13

11

13

13

0

DENV-3

10

9

10

10

0

DENV-4

12

11

12

12

0

JEV

14

12

14

14

0

Influenza viruses**

12

0

0

0

nd

Hantaviruses**

12

0

0

0

nd

  1. *A given primer set in the RT-LAMP unit was used to amplify other viral templates. ** These viruses were amplified with the primer sets of the five flaviviruses, individually. nd, not done. DENV, dengue virus; JEV, Japanese encephalitis virus; RT-PCR, reverse-transcription polymerase chain reaction; RT-LAMP, reverse-transcription loop-mediated isothermal amplification.