OVA-specific splenic CD8+T cell responses 10 days after replicon immunization in wild type and IRF3KO mice. The CD8+ T cell responses were measured in wild type (black circles (●)), IRF3 KO (open circles (○)) and naïve (black squares (■)) mice, 10 days post-immunization. The numbers of OVA-specific CD8+ T cells were investigated by IFNγ ELISpot. Data are pooled from four experiments, with 5 to 10 mice per group. Values are expressed as numbers of IFNγ SFC per million splenocytes. Each symbol represents an individual mouse and the group median values are indicated by bars. The statistical difference between the groups were p < 0.05 (*).