OVA-specific CD8+T cells in spleen 10 days after replicon immunization in wild type, TLR3KO and MyD88KO mice. The CD8+ T cell responses were measured in wild type, TLR3 KO (A) and MyD88 KO (B) mice. The numbers of OVA-specific CD8+ T cells were measured by IFNγ ELISpot in wild type (black circles (●)), KO (open circles (○)) and naïve (black squares (■)) mice. Data were pooled from two experiments with 3 to 5 mice per group (A) and from three experiments with 5 to 10 mice per group (B). Values are expressed as numbers of IFNγ SFC per million splenocytes. Each symbol represents an individual mouse and the group median values are indicated by bars. No statistical difference was detected between the groups of mice.