Figure 4

Subcellular localization of UPR sensor proteins ATF6 and p-PERK. MA104 cells were grown on glass coverslips and then infected with OSU or RRV at a MOI of five pfu/cell for seven hours. Mock infected cells treated with 2DG served as a positive control for UPR activation. A) ATF6 and B) p-PERK were stained with specific antibodies, followed by indicated Alexa-conjugated secondary antibodies. C) Epifluorescence microscopy of p-PERK expression over time during OSU infection (1-5 hpi) in MA104 cells.