Figure 3

Expression levels and subcellular localization of GRP78 and GRP94 proteins. A) GRP78 expression levels in mock infected cells, OSU infected cells, IFN treated cells, or cells treated with IFN prior to infection as measured by immunoblot. 60 ug of protein from lysates prepared for 2D-DIGE were electrophoresed on SDS-polyacrylamide gels, and proteins were transferred to nitrocellulose. Membranes were probed with anti-GRP78 and anti-actin as a loading control. B) GRP78 expression in OSU, NCDV (bovine) and RRV-infected cells 7 hours post infection. C) GRP78 and D) GRP94 localization was performed in MA104 cells grown on glass coverslips. The cells were infected with OSU or RRV at a MOI of 5 pfu/cell. At 7 hpi, the cells were fixed stained with anti-GRP78 or anti-GRP94. Nuclei were labeled with DAPI.