Figure 6
From: The heterogeneous ribonuclear protein C interacts with the hepatitis delta virus small antigen
Silencing of endogenous hnRNPC reduces expression of HDAgs. (A) Western blot analysis of Huh7 cells transfected with plasmid pSVD3, and pSIREN-RetroQ constructs. Knockdown of the hnRNPC protein was performed using a specific siRNA in Huh7 cells. 48 hrs after addition of puromycin, total protein extracts were prepared, separated by SDS-PAGE, and transferred to a nitrocellulose membrane. Blots were incubated with anti-hnRNPC, anti-clathrin or anti-HDAg antibodies. The displayed images are representative of three independent experiments. (B) Images were digitalized and band intensity was determined using the ImageJ program. The relative amounts of protein were obtained after normalization with the housekeeping protein clathrin. Inhibition of HDAg expression is presented as a percentage after comparison with the values obtained with the negative control vector pSIREN-RetroQ/Luc provided by the manufacturer.