Figure 5

Identification of S-HDAg domains involved in the S-HDAg/hnRNPC interaction, by yeast two-hybrid analyses. (A) Schematic representation of the deletion constructs of S-HDAg cloned in the bait vector pAS2-1. OLIG, NLS and RNA-BD represent the oligomerization, nuclear localization signal or RNA binding domains, respectively, identified in S-HDAg. (B) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids leucine or tryptophan (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or/and pAS2-1 plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking leucine, tryptophan, histidine and adenine, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-α-Gal).