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Figure 2 | Virology Journal

Figure 2

From: The heterogeneous ribonuclear protein C interacts with the hepatitis delta virus small antigen

Figure 2

Identification of hnRNPC as an S-HDAg interactor in a yeast two hybrid screening. (A) Schematic representation of hnRNPC and the GAL4AD fusion proteins identified in yeast positive clones (GAL4AD/hnRNPC and GAL4AD/hnRNPC1-108). GAL4AD/hnRNPC and GAL4AD/hnRNPC1-108 were identified in four and one positive clones, respectively. GAL4AD/hnRNPC1-108 codes for a truncated form of hnRNPC, that contains only the first 108 aminoacids. Both proteins contain a 5'untranslated region of hnRNPC mRNA (black bar). GAL4AD represents the activation domain of the GAL4 transcription factor and RRM and CC represent the RNA recognition or coiled-coil motifs, respectively, present in hnRNPC. (B) Western blot analysis of hnRNPC expression in yeast positive clones. Yeast extracts were prepared, separated on 12% SDS/PAGE gels, and immunoblotted with an anti-hnRNPC antibody. The positive clone expressing GAL4AD/hnRNPC1-108 was used in this analysis and AH109 yeast extracts were used as negative control.

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