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Table 2 Detection of Pbo-likeV in pig samples using conventional PCR and TaqMan real-time PCR.

From: Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV)

Animal

Health status

Type of tissue or samples

No. positive/no. tested samples (%)

   

Conventional PCR

Real-time PCR

 

Healthy

Serum

6/50 (12.0)

8/50 (16.0)

  

Tonsils

4/28 (14.3)

5/28 (17.9)

  

Total

10/78 (12.8)

13/78 (16.7)

Pigs

Diseased and

deceased

Serum

57/110 (51.8)

61/110 (55.5)

  

Tonsils

8/23 (34.8)

9/23 (39.1)

  

Lymph

6/11 (54.5)

6/11 (54.5)

  

Lung

22/36 (61.1)

25/36 (69.4)

  

Total

93/180 (51.7)

101/180 (56.1)

 

All

Total

103/258 (39.9)

114/258 (44.2)