Figure 2
From: Infectious alphavirus production from a simple plasmid transfection
Production of Infectious Sindbis Viruses. A) P0 viral titers from plasmid transfected BHK cells. 125 ng of plasmid DNA transfected with Lipofectamine 2000 reagents. Media samples were collected at the indicated times and viral titers were determined by plaque assays as plaque forming units (PFU)/ml. B) P1 viral titers in BHK cells. BHK cells were infected at MOI = 0.01 with 48 hr P0 viruses, then samples were collected at the indicated times and viral titers were determined by plaque assay.