Figure 3From: Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosisAlignment of deduced amino acid sequences of GPRPs in diverse plants. The GPRP sequences of Oryza sativa (OsGPRP) [DDBJ:NM_001061018], Sporobolus stapfianus (SsGPRP) [DDBJ:AJ242804] [16], broad bean (k158/VfGPRP, this study) [DDBJ:AB615379], Medicago truncatula (MtGPRP) [DFCI Medicago Gene Index TC:TC146460], Glycine max (GmGPRP) [DDBJ:BT089628], Arabidopsis thaliana (AtGPRP2), DDBJ:NM_118040], A. thaliana (GPRP) [DDBJ:NM_123903] [14], Populus trichocarpa (PtGPRP) [DDBJ:XM_002328813], and Picea sitchensis (PsGPRP) [DDBJ:EF083838] were aligned using the Clustal × program. The lined sequence was the central hydrophobic domain tentatively important for anchoring GPRP to a membrane [14]. A k158/VfGPRP mutant (VfGPmycRP) used in Figure 4 was made by inserting a Myc tag sequence, EQKLISEEDL, into the position indicated by a Myc-labeled arrowhead.Back to article page