Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells

Figure 6

Ectopic expression of miR-155 inhibited HBV X gene expression in human hepatoma cells. (A) 0.5 μg PAAV/HBV1.2 plasmid was transfected into HepG2 and HepG2-miR155 cells, or 0.5 μg PAAV/HBV1.2 plasmid and 50 nM miR-155 mimics/control RNA were cotransfected into HepG2 cells. 48 h later, RT-PCR analysis of HBV X gene (HBx) expression was performed. (B) Relative density analysis of HBx expression. (C) 0.5 μg PAAV/HBV1.2 plasmid and 50 nM miR-155 mimics/control RNA were cotransfected into HepG2 cells. 24 h after transfection, cells were treated with IFN-α2α at the concentration of 500 U/mL for another 24 h, and then RNA was extracted and RT-PCR analysis of HBx expression was performed. (D) Relative density analysis of HBx expression for (C). One representative of three independent experiments was shown. (*p < 0.05 by comparison with control cells).

Back to article page