Figure 5

Augmented type I IFN signaling by miR-155 over-expression in human hepatoma cells. (A) HepG2 cells transfected with 50 nM miR-155 mimics/control RNA were stimulated by 500 U/mL IFN-α2α for 12 h. RT-PCR analysis was performed to evaluate IFN-inducible genes. (B) Relative density analysis of the IFN-inducible genes. (C) SOCS1, p-STAT1 and p-STAT3 protein levels in cells above were tested by western blot analysis. (D) Relative density analysis of the signaling transduction protein levels in HepG2 cells. One representative of three independent experiments was shown.