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Figure 3 | Virology Journal

Figure 3

From: Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells

Figure 3

Over-expression of miR-155 enhanced IFN-Inducible genes expression in human hepatoma cells. HepG2 cells, HepG2-miR155 cells or HepG2 cells and H7402 cells transfected with 50 nM miR-155 mimics/control RNA were harvested. As described in Materials and Methods, (A, C) RT-PCR analysis was performed to evaluate IFN-inducible genes. (B, D) Relative density analysis of IFN-induced gene mRNA levels. (E, F) RT-PCR analysis and relative density analysis of IFN-inducible genes in H7402 cells 48h after transfection. (G, H) Western blot analysis of ISG15 levels in HepG2 cells treated as above. One representative of three independent experiments was shown. (*p < 0.05 by comparison with control cells).

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