Figure 3
From: Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells
Furin inhibiton assay on Ac-E3E2 and Ac-E3E2ΔTM infected Sf 21 cells. Sf21 cells were infected with Ac-E3E2 and Ac-E3E2ΔTM in the absence (-) or presence (+) of furin inhibitor. The cell fraction (A) and the concentrated medium fraction (B) were analyzed by western blotting using α-E2 polyclonal antiserum. Glycosylated proteins are indicated with an asterisk.