Figure 1

CHIKV E1 and E2 expression using recombinant baculoviruses. A) Schematic representation of four CHIKV glycoprotein constructs expressed from recombinant baculoviruses. The shaded areas represent transmembrane domains or signal peptides (ss), asterisks indicate predicted N-glycosylation sites, S and F indicate signalase and furin cleavage sites, respectively. The predicted molecular mass of the glycoproteins is indicated. All constructs were equipped with a C-terminal 6 × His-tail. B) Gene expression in the cell and in medium fractions of infected Sf21 cells was analyzed by Coomassie brilliant blue-staining and western blotting using α-His mabs and rabbit α-E1 and rabbit α-E2 polyclonal antisera.