Figure 7

Confocal microscopy showing the nuclear translocation of Stat1, Stat2 and Stat3-GFP in sensitive and resistant Huh-7 cells after IFN-α treatment. IFN-sensitive (S-5/15) and IFN-resistant (R-17/3)) cells were transfected with plasmid containing the Stat1-GFP, Stat2-GFP or Stat3-GFP. IFN-α 2 b (1000 IU/ml) was added after 48-hours of transfection and images were captured after 30 minutes of treatment. The nuclear translocation of each STAT-GFP construct with or without IFN-α treatment in the sensitive and resistant cells was examined under a confocal microscope. The images are represented as the superimposition of Green Fluorescent Protein (green), To-Pro3 633 (far red).