Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Mechanism of HCV's resistance to IFN-α in cell culture involves expression of functional IFN-α receptor 1

Figure 6

Stable expression of IFNAR1 restored the phosphorylation Stat proteins in the cured resistant Huh-7 cell line (R-17/3). The cured Huh-7 cell line (S-5/15) and (R-17/3) in culture were treated either with IFN-α 2 b (1000 IU/ml) or IL6 (50 ng/ml) and cells were harvested after 30 and 60 minutes. Ten micrograms of protein lysate was run on a NuPAGE 4-12% gel and immunoblotting was used to determine pStat1, pStat2 and pStat3 levels. (A) Phosphorylation of Stat1, Stat2 and Stat3 proteins in the sensitive and resistant Huh-7 cells. Left panel shows that IFN-α induced phosphorylation Stat1, Stat2, Stat3 proteins in S-5/15 cells. Middle panel shows the lack of phosphorylation of Stat1 and Stat2 in the R-17/3 cell line after treatment with IFN-α. Right panel shows that the stable transfection of R-17/3 cells with IFNAR1 restores the Stat1 and Stat2 phosphorylation by IFN-α treatment. Total Stat1 and Stat2 levels were indistinguishable. (B) Shows that IL-6 induced phosphorylation of Stat1 and Stat3 in Jak-Stat sensitive and defective Huh-7 cells.

Back to article page