Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Mechanism of HCV's resistance to IFN-α in cell culture involves expression of functional IFN-α receptor 1

Figure 2

Western blot analysis of Jak-Stat signaling between S-5/15 and R-17/3 cell line. An equal number of S-5/15 and R-17/3 Huh-7 cells were treated with IFN-α 2 b (1000 IU/ml). Cells were harvested at different time intervals (in hrs) by trypsin-EDTA treatment, washed in PBS. Western blot analysis was performed using antibodies to IFNAR1, IFNAR2, pJak1, Jak1, pTyk2, Tyk2, pStat1, Stat1, pStat2, Stat2 and β-actin and then blots were developed by ECL kit. (A) Expression level of Jak-Stat proteins between the two cell lines. Left panel shows the expression of Jak-Stat signaling proteins in the IFN-α sensitive Huh-7 cell line (S-5/15). Right panel shows the expression of Jak-Stat signaling proteins in the IFN-α resistant Huh-7 cell line (R-17/3). The level of β-actin protein in the blot indicated that equal amounts of protein lysate were loaded onto the gel in the Western analysis. (B) Show the expression of fully glycosylated mature form of IFNAR1 (~100 kD) and IFNAR2 (~90 kD) in all nine of different IFN-α resistant cell lines and one sensitive Huh-7 cell line (S-5/15) by Western blot analysis.

Back to article page