Human cell types important for Hepatitis C Virus replication in vivo and in vitro. Old assertions and current evidence

Table 4 Analysis of RT-PCR studies that detected HCV negative strand RNA.

		Studies
Cell types or tissue	Regular PCR	Corrected PCR	rTth PCR	Totals
L = liver	6/6	13/13	13/13	32/32
S = serum	3/6	6/11	3/6	12/23
P = plasma	2/4	1/3	0/1	3/8
PBMC	10/11	14/16	12/15	36/42
PBML	3/3	2/2	0/1	5/6
B = B cells/lymphocytes	5/5	3/4	3/3	11/12
T = T cells/lymphocytes	1/4	7/11	2/2	10/17
M = Monocytes/Macrophages	1/2	3/4	2/2	6/8
DC = Dendritic cells	1/1	1/1		2/2
BM = Bone marrow	2/2	0/1	2/2	4/5
Granulocytes, PMNG		2/2		2/2
Oral tissues	3/3		1/1	4/4
Carotid plaque			1/1	1/1
Skin			1/2	1/2
Lymph nodes			2/2	2/2
Pancreas			1/1	1/1
Spleen			1/1	1/1
Thyroid			1/1	1/1
Adrenal			1/1	1/1
CNS, brain, nerve		0/1	1/1	1/2
Muscle		0/1		0/1
NK	0/2			0/2
Total for all tissues	37/47	53/72	47/56	137/175
Percent	78.7	73.6	83.9	78.3
Total extrahepatic tissues	31/41	40/59	34/43	105/143
Percent	75.6	67.8	79.1	73.4
Studies with > = 1 positive result	21/21	22/23	28/30	71/75
Studies with > = 1 positive extrahepatic result	20/21	18/21	19/25	57/67

Data from Tables 1 through 3 have been compiled for numeric comparisons between the three versions of RT-PCR as well as overall results for each tissue or cell type.

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