Table 2 HCV negative strand RNA detection by corrected RT-PCR in chronic HCV-infected individuals

Acute, chronic

RNase A

Chronic: 6/12 PBMC; Incubation and acute 0 PBMC (no + or - strands)

[164]

Acute, chronic, IFN α-treated

Heat for 30 min, Core

14/35 chronic PBMC, 1/19 acute PBMC

[165]

Different genotypes

Core

3/11 PBMC, 3/10 CD15+ granulocytes;

[166]

1/10 CD14+ M, 2/9 CD19+ B, 0/7 CD3+ T

IFN α-treated

Heat 60 min

Untreated 0/18 S, 13/15 PBML; treated 0/7 S, 3/3 PBML

[167]

IFN α-treated

Heat 30 min

Untreated: 16/17 L, 5/14 S, 7/13 PBMC;

[168]

Treated, Nonresponders 9/10 L, 3/15 S, 3/7 PBMC

LT

Heat 1 hr, Core, 3 RT tested

16/20 L, 0/32 S, 2/26 PBMC, 0/6 BM

10 to 100 PBMC

[96]

LT

Core and tag

5/5 L

3 to 340 L

[95]

Mothers

Heat 60 min, RNase, low RT

5/13 PBMC that transmitted to child; 0/13 that didn't transmit HCV

[30]

Occult

Heat 30 min

2/5 PBMC

[169]

Peripheral neuropathy

Tag

0/9 muscle, 0/3 nerve

[170]

Chronic

Chemically modify 3' of RNA

9/10 L, 5/10 S, 1/10 PBMC

[171]

Chronic

Heat 2 hr, RNase H

0/106 P; 33/83 PBMC (40%)

[172]

Chronic

Heat 98 2 hr

0/7 P, 3/7 PBMC

1 to 100 PBMC

[173]

Chronic

Core, RNase, Heat > 20 min

4/8 PBMC

[174]

Chronic

Core and tag

8/10 L, 0/65 S or P, 1/30 PBMC, 0/12 LT PBMC

[175]

Chronic

Tag

L 2/2, 1/6 P, 1/6 DC, 0/6 CD4+, CD8+, CD14+ M, CD19+ B

300-600 L

[176]

Chronic

Tag

29/29 S, 11/29 PBMC, 6/29 PMNG

[177]

Chronic

Tag

3/3 L, 0/3 S

100 L

[178]

Chronic

Heat 45 min

43/45 L, 0/45 S, 37/45 PBMC

[179]

Chronic

Tag, RNase A, RNase H

1/1 L

60 L

[180]

Chronic

NS5

Chronic: 8/8 UTr, 1/3 Tr PBMC; Resolved: 1/10 UTr, 1/1 Tr PBMC;

up to 1000

[58]

Chronic: 6/8 UTr, 3/4 Tr CD4+ T; 6/8 UTr, 1/7 Tr CD8+ T;

Resolved: 6/10 Tr CD4+ T; 1/3 UTr, 2/4 Tr CD8+ T;

Chronic: 3/4 UTr B; 3/4 UTr M; Resolved: 4/5 UTr B; 2/3 UTr M

Chronic

Heat 30 min

13/33 S

[16]

Chronic

Heat 30 min

9/9 L, 0/9 S, 0/9 PBMC

[181]