Table 1 Primers used in construction of DNA clones.
DNA clones | Primer | Primer Sequences (5'-3')c | Primer location | Application |
|---|---|---|---|---|
PCV2 | Ps1 | GCGAATTC AACCTTAACCTTTCTTATTCT | 1418-1446 nt | PCV2 genome |
DNA clone | Ps2 | AAGAATTC TGGCCCTGCTCCC | 1407-1427 nt | Â |
| Â | a Ps3 | GCTCTAGA ATAACAGCACTGGAG | 1356-1378 nt | Duplicate |
| Â | a Ps4 | GAGGTACC CGTTTTCAGATATGAC | 1730-1753 nt | Â |
PCV1 | Pj3 | ACGGTACC CGAAGGCCGATTTGAA | 914-937 nt | PCV1 genome |
DNA clone | Pj4 | TAGGTACC TCCGTGGATTGTTCTCC | 899-923 nt | Â |
| Â | bEcoRV-f12 | GCGATATC ATGGAGAAGAAGTTGTTGT | 649-675 nt | Duplicate |
| Â | bEcoRI-r12 | CCGAATTC TCTTTCACTTTTATAGGATG | 1721-1748 nt | Â |
PCV1-NLS2 | ClaI-f | CAATCGAT AACGCCTCCTTGGATACGTCATC | 1694-1724 nt | PCV1 ΔNLS |
DNA clone | BglII-r | GGAGATCT TCAATTCCCGCCTTTCTAG | 1566-1592 nt | Â |
| Â | BamH-F | TAGGATCCCCGTGCCATTTTTCCTTC | 1594-1619 nt | PCV2 NLS |
| Â | MspI-r | CGTTACCGG AGAAGAAGACAC | 1696-1716 nt | Â |
PCV2-NLS1 | BalI-PCV2-F | CCTTGGCCA CGTCATATCTGAA | 1720-1741 nt | PCV2 ΔNLS |
DNA clone | BamH-PCV2-R | GAAAAATGGGATCC TCAACACC | 1594-1615 nt | Â |
| Â | BglII-PCV1-F | TACAGATCT TTCGGCGCCA | 1588-1606 nt | PCV1 NLS |
| Â | BalI-PCV1-R | TATTGGCCA AGGAGGCGTTA | 1701-1720 nt | Â |