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Figure 5 | Virology Journal

Figure 5

From: Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication

Figure 5

Synchronized PK-15 cells were infected with viruses all at an MOI of 0.1. (A)The virus loads for PCV1, PCV2, PCV1-NLS2 and PCV2-NLS1 in cell cultures at each time point were determined by real-time PCR based on PCV1 or PCV2 ORF2 gene. (B) Kinetics of viral capsid protein (28 kD) biosynthesis of progeny viruses from infected PK-15 cells at different time points were confirmed by western blot analysis with polyclonal antibodies to PCV1 or PCV2 capsid protein.

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