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Figure 3 | Virology Journal

Figure 3

From: Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication

Figure 3

In vitro viability of progeny viruses after initial transfection of PK-15 cells with DNA clones. Synchronized PK-15 cells were transfected with 20 μg of PCV1, PCV2, PCV1-NLS2 and PCV2-NLS1 DNA clone followed by twenty-six serial passages. The DNA clones were capable of producing infectious and viable virions in vitro, and all the progeny viruses gave rise to gradually increasing infectious titers during passages and propagated stably after 16 to 20 passages. The infectious titers were determined at each passage according to the Reed-Muench method.

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Virology Journal

ISSN: 1743-422X