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Figure 1 | Virology Journal

Figure 1

From: Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication

Figure 1

Schematic construction of the infectious DNA clones. (A) The PCV1 DNA clone was constructed by ligating a duplicated fragment (915-1740 nt) to the Kpn I-digested PCV1 genome in pUC-18. The chimeric PCV1-NLS2 DNA clone was constructed replacing the ORF2 NLS sequence in PCV1 genomic backbone with NLS of PCV2 ORF2. (B) The PCV2 DNA clone was constructed by ligating a fragment (1419-1745 bp) to the Eco R I-digested PCV2 genome in pUC-18. The chimeric PCV2-NLS1 was constructed by replacing the PCV2 ORF2 NLS with PCV1 ORF2 NLS. The partial duplications were indicated by dashed. Positions of primers and the directions of ORF were marked by arrows and the orientations of the triangles, respectively.

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Virology Journal

ISSN: 1743-422X