Figure 4
From: HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
The interaction of HuR with the 3' UTR conserved in CSFV strains irrespective of virulence. The 3' UTR of CSFV strains of varied virulence bind to GST-HuR in vitro. Autoradiogram of EMSA of the 32P labeled RNA of the 3' UTR belonging to four different CSFV strains ALD, LPC, 93KS, and 96TD run on a 5% non-denaturing polyacrylamide gel. Free probe derived from each strain is loaded in lanes 1, 5, 9, and 13. The purified GST only as the control is loaded in lanes 2, 6, 10, and 14; and GST-HuR as the binding partner (lanes 3, 7, 11, and 15) and anti-HuR antibody are included in the super-shift reactions (lanes 4, 8, 12, and 16) indicated above the lanes. Arrows indicate the positions of the free, shifted, and super-shifted radiolabeled probes.