Figure 3
From: HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus
The specific interaction between HuR and the 3' UTR of CSFV RNA. GST-HuR binds to CSFV ALD 3' UTR. An autoradiogram of electrophoretic mobility shift assay (EMSA) runs on a 5% non-denaturing PAGE. (A) Free probe (32P labeled CSFV ALD3'UTR) without any proteins is loaded in lane 1, 1 μg BSA in lanes 2 and 3, and GST-HuR in lanes 4 and 5 are used in an EMSA. The extra1 μg of anti-HuR antibody is included in lanes 3 and 5. (B) EMSA with unlabeled homo-polyribonucleotides. Free probe without protein components is loaded in lane 1, about 1 μg each of GST-HuR and anti-HuR antibody are loaded with equal mass cold homo-polyribonucleotides, polyU in lane 2, polyG in lane 3, polyC in lane 4, and polyA in lane 5. Arrows indicate the positions of the free, shifted, and super-shifted radiolabeled probes.