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Table 2 Cytopathic effect of viruses in BVK168 cells.

From: A new permanent cell line derived from the bank vole (Myodes glareolus) as cell culture model for zoonotic viruses

BVK168 passage

days p.i.

VSV

VACV

CPXV

SINV

PIXV

INKV

USUV

1st

1

+

-

-

-

-

-

-

 

2

+++

-

(+)

-

-

-

++

 

3

++++, cf

-

++++, cf

(+)

(+)

-

++++, cf

 

4

cf

(+)

cf

+

+

(+)

cf

 

5

cf

(+)

cf

++

++

(+)

cf

 

6

cf

(+)

cf

+++

+++

++++, cf

cf

 

7

cf

++, cf

cf

++++, cf

++++, cf

cf

cf

2nd

1

+++

-

-

-

-

-

-

 

2

++++, cf

-

(+)

(+)

-

-

++

 

3

cf

++

++

+

(+)

(+)

++++, cf

 

5

cf

+++

++++, cf

++

+

+

cf

 

5

cf

+++

cf

+++

++

++

cf

 

6

cf

++++, cf

cf

++++, cf

+++

+++

cf

 

7

cf

cf

cf

cf

++++, cf

++++, cf

cf

  1. Abbreviations: p.i., post infectionem; VSV, vesicular stomatitis virus strain India; VACV, vaccinia virus strain Munich 1; CPXV, cowpox virus strain 81/01; SINV, Sindbis virus strain C377; PIXV, Pixuna virus strain BeAr35645; INKV, Inkoo virus strain TN-98-5085; USUV; Usutu virus strain 0679/2006; -, no CPE; (+), 10-20% visible CPE; +, 20-40% visible CPE; ++, 40-60% visible CPE; +++, 60-80% visible CPE; ++++, >80% visible CPE; cf, culture frozen until further use
  2. Viral cytopathic effect in BVK168 cells were evaluated semi-quantitative. Puumalavirus and Borna disease virus are not listed as these showed no visible CPE and evidence for infection of cells by these viruses had to be confirmed by real-time RT-PCR and/or immunofluorescence assay.