BVK168 passage | days p.i. | VSV | VACV | CPXV | SINV | PIXV | INKV | USUV |
---|
1st |
1
| + | - | - | - | - | - | - |
|
2
| +++ | - | (+) | - | - | - | ++ |
|
3
| ++++, cf | - | ++++, cf | (+) | (+) | - | ++++, cf |
|
4
| cf | (+) | cf | + | + | (+) | cf |
|
5
| cf | (+) | cf | ++ | ++ | (+) | cf |
|
6
| cf | (+) | cf | +++ | +++ | ++++, cf | cf |
|
7
| cf | ++, cf | cf | ++++, cf | ++++, cf | cf | cf |
2nd |
1
| +++ | - | - | - | - | - | - |
|
2
| ++++, cf | - | (+) | (+) | - | - | ++ |
|
3
| cf | ++ | ++ | + | (+) | (+) | ++++, cf |
|
5
| cf | +++ | ++++, cf | ++ | + | + | cf |
|
5
| cf | +++ | cf | +++ | ++ | ++ | cf |
|
6
| cf | ++++, cf | cf | ++++, cf | +++ | +++ | cf |
|
7
| cf | cf | cf | cf | ++++, cf | ++++, cf | cf |
- Abbreviations: p.i., post infectionem; VSV, vesicular stomatitis virus strain India; VACV, vaccinia virus strain Munich 1; CPXV, cowpox virus strain 81/01; SINV, Sindbis virus strain C377; PIXV, Pixuna virus strain BeAr35645; INKV, Inkoo virus strain TN-98-5085; USUV; Usutu virus strain 0679/2006; -, no CPE; (+), 10-20% visible CPE; +, 20-40% visible CPE; ++, 40-60% visible CPE; +++, 60-80% visible CPE; ++++, >80% visible CPE; cf, culture frozen until further use
- Viral cytopathic effect in BVK168 cells were evaluated semi-quantitative. Puumalavirus and Borna disease virus are not listed as these showed no visible CPE and evidence for infection of cells by these viruses had to be confirmed by real-time RT-PCR and/or immunofluorescence assay.