Table 4 The reaction system of RT-LAMP assay

10 × Thermopol Buffer

2.5 μL

MgSO₄

5 mmol/L

Betaine

1 mmol/L

F3

0.2 μmol/L

B3

0.2 μmol/L

primer

FIP

1.6 μmol/L

BIP

1.6 μmol/L

LF

0.8 μmol/L

LB

0.8 μmol/L

dNTP

1.4 mmol/L

Calcein

25 μmol/L

MnCl₂

0.5 mmol/L

M-MuLV RTase

200U

Bst DNA polymerase

8U

Template RNA

2 μL

Nuclease-free water

Up to 25 μL