Table 2 Primers and Probes used to Evaluate Immunomodulator and Virus Expression
From: Immunomodulator expression in trophoblasts from the feline immunodeficiency virus (FIV)-infected cat
Gene | Primer | Primer Sequence (5' to 3') | Primer Length | Probe Sequence (5' to 3') | Accession # or Reference |
|---|---|---|---|---|---|
β actin | Forward | GACTACCTCATGAAGATCCTCACG | 24 | ACAGTTTCACCACCACCGCCGAGC | AB051104 |
| Â | Reverse | CCTTGATGTCACGCACAATTTCC | 23 | Â | Â |
IL-1β | Forward | ATTGTGGCTATGGAGAAACTGAAG | 24 | TTTGCCTGCTCACAACCCCTCCAG | M92060 |
| Â | Reverse | TCTTCTTCAAAGATGCAGCAAAAG | 24 | Â | Â |
IL-6 | Forward | GTGTGACAACTATAACAAATGTGAGG | 26 | CAAGGAGGCACTGGCAGAAAACAACCT | L16914 |
| Â | Reverse | GTCTCCTGATTGAACCCAGATTG | 23 | Â | Â |
IL-10 | Forward | ACTTTCTTTCAAACCAAGGACGAG | 24 | TCTCGGACAAGGCTTGGCAACCCA | AF060520 |
| Â | Reverse | GGCATCACCTCCTCCAAATAAAAC | 24 | Â | Â |
IL-12p35 | Forward | ACACCAAGCCCAGGAATGTTC | 21 | AACCACTCCCAAACCCTGCTGCGA | U83185 |
| Â | Reverse | TGGCCTTCTGAAGCGTGTTG | 20 | Â | Â |
IL-12p40 | Forward | GAAGTACACAGTGGAGTGTCAGG | 23 | CAGTGCCTGCCCGGCTGCCG | U83184 |
| Â | Reverse | GGTTTGATGATGTCCCTGATGAAG | 24 | Â | Â |
CD134 | Forward | CAGGTTATGGGATGGAGAGTCG | 22 | TGACCAGGACACCAAGTGCCTCCAGTG | AY738589 |
| Â | Reverse | TGCAAGGCTCGTAGTTCACG | 20 | Â | Â |
CXCR4 | Forward | AAGGCAGTCCATGTCATCTACAC | 23 | ACCTCTACAGCAGTGTCCTCATCCTGGC | AJ009816 |
| Â | Reverse | AGACCACCTTTTCAGCCAACAG | 22 | Â | Â |
SDF-1α | Forward | GCTACAGATGTCCTTGCCGATTC | 23 | TCGAGAGCCACGTTGCCAGAGCCA | AB011965 |
| Â | Reverse | TCTTCAGCCTCGCCACGATC | 20 | Â | Â |
IL-2 | Forward | ACGGTTGCTTTTGAATGGAG | 20 | CCCCAAACTCTCCAGGATGCTCA | Ref 23 |
| Â | Reverse | CAATTCTGTGGCCTTCTTGG | 20 | Â | Â |
IL-4 | Forward | CCCCTAAGAACACAAGTGACAAG | 23 | TTCTGCAGAGCCACAACCGTGC | Ref 23 |
| Â | Reverse | CCTTTGAGGAATTTGGTGGAG | 21 | Â | Â |
IL-5 | Forward | TGCTTCTGCATTTGAGTTTG | 20 | TGGCAGAAACATAGGCAGCCCC | Ref 23 |
| Â | Reverse | CAGCCTATTCATGGGACTTTG | 21 | Â | Â |
IL-18 | Forward | GGAGATCAACCTGTGTTTGAGGAT | 24 | ATTCTGACTGTACAGATAATGCACCCCGGAC | Ref 25 |
| Â | Reverse | GATGGTTACTGCCAGACCTCTAGTG | 25 | Â | Â |
GM-CSF | Forward | AATGAAACGGTAGAAGTCGTCTCTG | 25 | TTGACCCTGAGGAGCCGAATTGCC | Ref 26 |
| Â | Reverse | CGTACAGCTTTAGGTGAGTCTGCA | 24 | Â | Â |
IFN-γ | Forward | TGGTGGGTCGCTTTTCGTAG | 20 | CATTTTGAAGAACTGGAAAGAGGAGAGTGATAAAACAAT | Ref 24 |
| Â | Reverse | GAAGGAGACAATTTGGCTTTGAA | 23 | Â | Â |
TNF-α | Forward | CTTCTCGAACTCCGAGTGACAAG | 23 | TAGCCCATGTAGTAGCAAACCCCGAAGC | Ref 26 |
| Â | Reverse | CCACTGGAGTTGCCCTTCA | 19 | Â | Â |
GAG | Forward | GTATGATCGTACTCATCCTCCTGAT | 25 | AGACCACTGCCCTACTTCACTGCCG | Ref 17 |
| Â | Reverse | TCTACATTGCATTCTGGCTGGT | 22 | Â | Â |