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Figure 5 | Virology Journal

Figure 5

From: Detection and characterization of spontaneous internal deletion mutants of Beet Necrotic yellow vein virus RNA3 from systemic host Nicotiana benthamiana

Figure 5

The detection of RNA3, RNA3α and RNA3αM2 in the systemic infected plants. The in-vitro transcripts of RNA3(A), RNA3α(B) and RNA3αM2 (C) were inoculated on the N. benthamiana plants respectively with total RNA including RNA1 and RNA2. In 14 dpi, the total RNA was extracted from systemic leave of each infected plant, and carried out for RT-PCR with the RNA2 or RNA3 specific primers. In each infected plant, the RNA2 detection as a positive control for successful infection. The plants infected by RNA1 2 were as negative control of RNA3 detection. (E) The symptom of infected N. benthamiana plants by BN12, RNA3α and RNA3αM2 in 15 dpi.

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Virology Journal

ISSN: 1743-422X