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Figure 1 | Virology Journal

Figure 1

From: Detection and characterization of spontaneous internal deletion mutants of Beet Necrotic yellow vein virus RNA3 from systemic host Nicotiana benthamiana

Figure 1

The characteristic of viral RNA3, 4 and 5 in N. benthamiana infected by Chinese BNYVV isolates. (A) The systemic symptom of isolates BN345, BN3 and BN3A on the N. benthamiana plants. The mock was inoculated by buffer. (B) The detection of RNA3, 4, 5 from the systemic leaves of the N. benthamiana plants by RT-PCR. Each lane represented an individual infected plant. The RNA from infected leaves of T.expansa was detected by RT-PCR as positive control (CK+ lane). The M (mock) was the result of RT-PCR from buffer infected N. benthamiana plants. The expected sizes of the PCR fragments were indicated on the right. (C) The northern detection of RNA3 and D-RNA3s from upper leaves of N. benthamiana individually infected by the isolate of BN3 and BN3A. (D) RNA5 detection by northern blot in the inoculated (ino) and systemic (sys) leave infected by BN12 or BN125.

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