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Table 2 Comparison of RMA and RT-rtPCR assays.

From: Real-world comparison of two molecular methods for detection of respiratory viruses

RMA (1)*

Virus

RT-rtPCR (1)**

Positive

Negative

Total

Kappa (95%CI)†

RSV

Positive

70

8

  
 

Negative

28

116

222

0.66 (0.56-0.76)

HRV

Positive

50

13

  
 

Negative

6

153

222

0.78 (0.68-0.87)

HMPV

Positive

2

0

  
 

Negative

6

214

222

 

Influenza A

Positive

4

21

  
 

Negative

3

194

222

 

PIV 1

Positive

9

4

  
 

Negative

1

208

222

 

PIV 2

Positive

1

0

  
 

Negative

0

221

222

 

CoV NL63

Positive

5

1

  
 

Negative

3

211

220

 

CoV OC43

Positive

4

1

  
 

Negative

0

215

220

 
 

RT-rtPCR (2)

    

RSV

Positive

95

12

 

0.86 (0.78-0.92)

 

Negative

3

110

220

 

HMPV

Positive

4

1

  
 

Negative

2

211

218

 

RMA (2)

 

RT-rtPCR (1)

Positive

Negative

  

Influenza A

Positive

5

20

  
 

Negative

0

196

222

 
  1. *Kappa for agreement assessments is reported when all cells are ≥ 3.
  2. RSV = respiratory syncytial virus, HRV = human rhinovirus, HMPV = human metapneumovirus, PIV = parainfluenza virus, CoV = coronavirus.
  3. RMA (1) = first RMA run, RMA (2) = second RMA run with revised primers/probes, RT-rtPCR (1) = first RT-rtPCR run, RT-rtPCR (2) = second RT-rtPCR run with revised primers/probes.