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Table 2 Comparison of RMA and RT-rtPCR assays.

From: Real-world comparison of two molecular methods for detection of respiratory viruses

RMA (1)*
Virus RT-rtPCR (1)** Positive Negative Total Kappa (95%CI)†
RSV Positive 70 8   
  Negative 28 116 222 0.66 (0.56-0.76)
HRV Positive 50 13   
  Negative 6 153 222 0.78 (0.68-0.87)
HMPV Positive 2 0   
  Negative 6 214 222  
Influenza A Positive 4 21   
  Negative 3 194 222  
PIV 1 Positive 9 4   
  Negative 1 208 222  
PIV 2 Positive 1 0   
  Negative 0 221 222  
CoV NL63 Positive 5 1   
  Negative 3 211 220  
CoV OC43 Positive 4 1   
  Negative 0 215 220  
  RT-rtPCR (2)     
RSV Positive 95 12   0.86 (0.78-0.92)
  Negative 3 110 220  
HMPV Positive 4 1   
  Negative 2 211 218  
RMA (2)
  RT-rtPCR (1) Positive Negative   
Influenza A Positive 5 20   
  Negative 0 196 222  
  1. *Kappa for agreement assessments is reported when all cells are ≥ 3.
  2. RSV = respiratory syncytial virus, HRV = human rhinovirus, HMPV = human metapneumovirus, PIV = parainfluenza virus, CoV = coronavirus.
  3. RMA (1) = first RMA run, RMA (2) = second RMA run with revised primers/probes, RT-rtPCR (1) = first RT-rtPCR run, RT-rtPCR (2) = second RT-rtPCR run with revised primers/probes.