Table 1 Primer and probe sequences, reaction concentrations, and annealing temperatures used for RT-rtPCR assays.

HRV (5' UTR)$

CY+AGCC+TGCGTGGC

0.8

GAAACACGGACACCCAAAGTA

0.8

TCCTCCGGCCCCTGAATGYGGC

0.1

60

[20]

1^st Round RSV (M)

GGAAACATACGTGAACAAGCTTCA

1

A: CATCGTCTTTTTCTAAGACATTGTATTGA

0.5

TGTGTATGTGGAGCCTTCGTGAAGCAAG

0.2

60

[19]

B: TCATCATCTTTTTCTAGAACATTGTACTGA

0.5

2^nd Round RSV (M)

GGCAAATATGGAAACATACGTGAA

1

TCTTTTTCTAGGACATTGTAYTGAACAG

1

CTGTGTATGTGGAGCCTTCGTGAAGCT

0.2

60

[18]

1^st Round HMPV (N)

CATATAAGCATGCTATATTAAAAGAGTCTC

1

CCTATTTCTGCAGCATATTTGTAATCAG

1

TGYAATGATGAGGGTGTCACTGCGGTTG

0.2

60

[21]

2^nd Round HMPV (N)

CATAYAARCATGCTATATTAAAAGAGTCTC

1

CCTATYTCWGCAGCATATTTGTAATCAG

1

CAACHGCAGTRACACCYTCATCATTRCA

0.2

60

*

HCoV OC43 (N)

CGATGAGGCTATTCCGACTAGGT

1

CCTTCCTGAGCCTTCAATATAGTAACC

1

TCCGCCTGGCACGGTACTCCCT

0.5

60

[22]

HCoV 229E (N)

CAGTCAAATGGGCTGATGCA

1

AAAGGGCTATAAAGAGAATAAGGTATTCT

1

CCCTGACGACCACGTTGTGGTTCA

0.5

60

HCoV-NL (N)

AGGACCTTAAATTCAGACAACGTTCT

1

GATTACGTTTGCGATTACCAAGACT

1

TAACAGTTTTAGCACCTTCCTTAGCAACCCAAACA

0.5

66

[17]

PIV 1 (N)

GGATATCCTGCATGTCTCGG

1.5

GGCACTTTTAACGGTTCCATC

1.5

CCATAACAAGTAGTGCTGGTCTAAGAAAAGGATTCTTC

0.2

60

*

PIV 2 (N)

CACTTAAATATGGACTTGGAACAAGATG

1.5

TCCATCAGYTTTGGTGATTCTAATAGAG

1.5

CTACATTATCAGAGTCTAGGACCCATGGCCAA

0.2

60

*

PIV 3 (N)

AGCCATGCAACAGTATGTGACG

1.5

TTRGMTTCGTGTGTCACTCCAAGTTC

1.5

ATYTGAGCTTCRGCATCACGTGCTACTG

0.2

60

*

Flu A (M)

GACCRATCCTGTCACCTCTGAC

0.8

AGGGCATTYTGGACAAAKCGTCTA

0.8

TGCAGTCCTCGCTCACTGGGCACG

0.2

55

§

Flu B (NS)

TCCTCAACTCACTCTTCGAGCG

0.8

CGGTGCTCTTGACCAAATTGG

0.8

CCAATTCGAGCAGCTGAAACTGCGGTG

0.2

55

§