Skip to main content

Table 1 Intra- and inter-assay reproducibility of the EV MGB/CLP (TMC-PCR) and TAMRA/NonCLP real time RT-PCR (TTN-PCR)

From: Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe

 

Intra assaya

Inter assayb

 

105TCID50/mL

104TCID50/mL

100 TCID50/mL

10 TCID50/mL

Assay

Meanc

S.Dd

Meanc

S.Dd

Meanc

S.Dd

Meanc

S.Dd

TTN-PCR e

21.22

0.24

24.82

0.16

29.05

1.89

32.62

2.09

TMC-PCR f

21.55

0.08

25.02

0.07

30.65

0.86

33.95

0.80

  1. aEach Ct value was determined from eight replicates within a assay
  2. bAssays were performed three times on different days in an independent manner
  3. cMean of threshold cycle (Ct) value
  4. dStandard deviation
  5. eTAMRA/NonCLP real time RT-PCR assay
  6. fMGB/CLP real time RT-PCR assay.