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Table 1 CDNA density obtained for Wild type, K65R+L74V and K65R+L74I RTs

From: A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Group of cDNA Bandsa

cDNA Density

 
 

WT

65R+74V

65R+74I

p-values

1-6

1153 ± 103.0

1125 ± 79.0

1152 ± 102.0

0.38b, 0.49c

7-12

1375 ± 80.5

1282 ± 82.5

1332 ± 72.5

0.237d

13-18

1545 ± 100.0

1309 ± 89.0

1549 ± 95.5

0.016d

19-24

1592 ± 94.5

1202 ± 102.5

1497 ± 70.5

0.007d

25-30

1521 ± 76.5

1059 ± 109.0

1321 ± 56.5

0.010d

31-36

1483 ± 76.5

854 ± 74.0

1018 ± 68.5

0.023d

37-42

1410 ± 46.0

655 ± 65.0

789 ± 109.5

 

43-48

1314 ± 55.0

572 ± 72.5

672 ± 82.0

0.00007e,.0001f

49-54

1254 ± 74.0

 

614 ± 84.0

 

55-60

962 ± 57.5

 

362 ± 62.5

 

61-66

727 ± 73.0

   

67-72

606 ± 102.0

   
  1. a Groups of cDNA bands from 6 μl lanes of three RTs shown above (Figure 5)
  2. b WT/K65R+L74V and WT/K65R+L74I, identical p values were obtained comparing both double mutants with WT
  3. c WT/K65R+L74I
  4. d K65R+L74V/K65R+L74I
  5. e WT/K65R+L74V
  6. f WT/K65R+L74I
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Virology Journal

ISSN: 1743-422X