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Figure 6 | Virology Journal

Figure 6

From: A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Figure 6

Quantification of cDNA bands synthesized by WT, K65R+L74V and K65R+L74I RTs. Groups of 6 bands from bottom to top of each lane were scanned and quantified by Intelligent Quantifier software (Bio Image Systems, Inc., Jackson, MI). The graph shows the cDNA density of bands obtained with 6 μl of RT lysates. RT containing K65R+L74I mutation showed a significant increase in the density of cDNA bands (13-36) in comparison to K65R+L74V RT.

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Virology Journal

ISSN: 1743-422X