Figure 4

The effect of viral proteins and cyclophilin inhibition on apoB secretion. A) Supernatants and whole cell lysates from equal numbers of sg-1b replicon and naïve Huh7 cells were used to quantify apoB protein by ELISA. The average apoB protein levels from two experiments was normalized to naïve Huh7 cells and graphed as fold change in apoB protein. B) Sg-1b replicon and Huh7 naïve cells were treated with varying concentrations of NIM811 for 48h. ApoB was measured in the supernatants by ELISA. The amount of apoB protein was normalized to DMSO controls for each cell type and plotted as relative secretion ± standard deviation from three experiments.