Figure 2

ApoB accumulates as crescents in the ER surrounding the lipid droplets of sg-1b replicon cells treated with NIM811. Sg-1b replicon cells were grown in the presence of compound for 24h, after which they were fixed, permeabilized and stained using antibodies specific for apoB, NS5A and PDI. BODIPY 493/503 was used to visualize lipid droplets, and DAPI for nuclei. A) ApoB (red) is dispersed throughout the secretory pathway in sg-1b cells grown in the presence of DMSO. Following growth in NIM811, apoB forms ring-like and crescent shaped structures surrounding the lipid droplets (green). B) In DMSO treated cells, some of the apoB protein in replicon cells is localized to the ER, as shown by the overlapping staining pattern with the ER marker PDI. The ring-like and crescent shaped structures containing most of the detectable apoB protein co-localize with PDI in the presence of NIM811.