Figure 5
From: Gallus Heat shock cognate protein 70, a novel binding partner of Apoptin
Localization of Apoptin and exogenous Hsc70. (A) Localization of exogenous Hsc70 in DF-1 cells. DF-1 cells were seeded on 24-well plates with coverslips and cultured overnight. The cells were transfected with pDsRed-Hsc70 plasmids. After 24 hours transfection, the cells were fixed with 1% paraformaldehyde. After washing, the fixed cells were permeabilized with 0.1% Triton X-100. Nuclei were counterstained with DAPI (Blue). (B) Colocalization of exogenous Hsc70 with Apoptin in the nucleus. DF-1 cells were transfected with a pEGFP-Apoptin plasmid and pDsRed-Hsc70 for 24 hours and treated as described above. The cell samples were observed with a laser confocal scanning microscope. The scale bars in white represent 10 μm.