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Figure 3 | Virology Journal

Figure 3

From: Gallus Heat shock cognate protein 70, a novel binding partner of Apoptin

Figure 3

Determination of Apoptin-binding domain for Gallus Hsc70. (A) Schematics represent the genes encoding full length and truncated Apoptin molecules (Δ1 through 4). The numbers indicate amino acid positions within the molecule. (B) Endogenous Hsc70 interacts with different truncated Apoptin molecules. DF-1 cells were transfected with full length Myc-Apoptin (WT) or various truncated Myc-Apoptin constructs (Δ1: 1-90aa; Δ2:1-60aa; Δ3: 30-121aa; Δ4: 60-121aa). Twenty-four hours after transfection, cell lysates were immunoprecipitated with anti-Myc monoclonal antibody and were analyzed by Western blot using anti-Myc or anti-Hsc70 antibodies. (C) Schematic representation of the Apoptin's binding domain (amino acids 30-60) for Hsc70.

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