Figure 1
From: Gallus Heat shock cognate protein 70, a novel binding partner of Apoptin
Yeast two-hybrid screening of Apoptin associated proteins. (A) Negative control: Yeast transformed with the β-galactosidase negative control plasmids (pGBKT7-Lam and pGADT7-T) did not turn blue. (B) Positive control: Yeast transformed with the β-galactosidase positive plasmids (pGBKT7-53 and pGADT7-T) turned blue within 20-30 min. (C)-(G) Experimental group: Yeast colonies co-transformed with the pGADT7-derivative plasmids and pGBKT7-Apoptin plasmids were checked periodically for color change.