Phylogenetic analysis of porcine parvoviruses from swine samples in China

Table 1 Primers used in the amplification of the PPV genome.

Primer pairs	Location ^a	Sequences (5'-3')	Annealing temperature (°C)	Amplicon length (bp)
A1	244-1497	CACTTCGCTCCAGAGACACAGCTA	58	1254
		TGTTGATGCTGGCCCATGAAATAG
A2	1388-2356	TCAGCATGCACAATTGGAACTACA	56	969
		GTTTTATATGTATGCCCACCACCC
A3	2214-3903	GGAAATAGAAACCGACATAAGAGC	55	1690
		TTATATTGTGTGTCTGCTGTTGGT
A4	3796-4456	AATTAGGCCAGCTCAGGTAGGATA	59	661
		TGTTGTTGTGTGTTGTTGAATAGG
A5	4239-4854	GACTACATGTTACAGCTCCATTTG	54	489-616 b
		ATAGTAAACACATGAGAGCTTGTT

^a The position of the primer pairs are based on the entire genome sequence of the PPV NADL-2 strain [GenBank: NC_001718].
^b The PCR products between 489-616 bp were all regarded as positive samples and sequenced, as a fragment deletion of 127 bp or less may occur in the amplification region of the A5 primer pair.

ISSN: 1743-422X