Differences in mutated region for the TGBp3 for isolates P19, P22 and DB1. First three proteins are in the surface representation, colored according to the distribution of the electrostatic surface potential calculated with ABPS (see Methods). Blue indicates positively charged regions, red indicates negatively charged regions. The fourth picture shows three superimposed regions - LEA for P19 (blue) and DB1 (yellow) isolates, LKA for P22 isolate (green). All figures have been drawn using PyMOL .