Multiple Sequence Alignment of NS4A protein of HCV genotype 1b and 1c. Position of different amino acid is represented by numerical numbers at the top of the figure. The isolate (genotype)/country-serial number of the sequences are shown at the left side of the figure. Dots and Roman letters in figure indicate similarity and amino acid substitutions respectively relative to the first sequence patient 2_28 reported from France. Numerical number on the right side of the figure indicates number of amino acid substitutions in individual sequences.