Figure 4
From: High level soluble expression, one-step purification and characterization of HIV-1 p24 protein
Western blotting analysis of the recombinant p24 protein. Lane 1: E. coli whole cell harboring empty plasmid pQE30 after induction with IPTG; Lane 2: E. coli whole cell harboring pQE30-p24 after induction with IPTG; Lane 3: cell extract of E. coli harboring pQE30-p24 after induction with IPTG (pellets); Lane 4: cell extract of E. coli harboring pQE30-p24 after induction with IPTG (supernatant); Lane 5: purified His-tagged p24 protein.