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Figure 5 | Virology Journal

Figure 5

From: Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: Case Report

Figure 5

Rapid diagnosis of acute Lassa fever virus infection by Lateral Flow Immunoassay (LFI) in patient G-1180 and contacts -A and -B. LFI tests contain a murine monoclonal antibody capture and an heterologous gold-conjugated antibody for the detection of LASV nucleoprotein (NP) in the serum of suspected Lassa fever patients. Twenty-five μL of serum were applied to the sample well in an LFI module and chased with 3 drops (~ 100 μL) of optimally formulated buffer. After 10 minutes the results were recorded photographically. A representative normal serum sample analysis from a Sierra Leonean donor (S.L. N01) is shown for comparison. Only the control line developed with this serum sample. Conversely, sera from G-1180 generated a detectable precipitate in the test line, indicative of LASV NP antigen. The LFI platform detected NP antigen from days 6 - 8. Days 9 and 10 show no detectable antigen in this format. Sera from G-1180 contacts -A and -B did not produce a detectable signal on LFI, despite detection of antigen by a sensitive NP ELISA (figure 4A).

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Virology Journal

ISSN: 1743-422X