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Table 3 G9R-NA OPXV assay sensitivity to North American OPXV DNA

From: Detection of North American orthopoxviruses by real time-PCR

Virus

DNA (fg)

200

20

10

5

2.5

1.25

0.625

Raccoonpox

Pure

33

36.5

37.9

38.9 (20)

39.4 (5)

NT

NT

Skunkpox

Pure

28.9

32.7

34

35

36

37.8 (18)

39.2 (11)

Volepox

Pure

30.4

33.9

35.2

36.1

37.4

38.8 (23)

39.4 (19)

  1. The average of the cycle when fluorescence crossed the threshold (Ct) for each positive sample is shown.
  2. NT, not tested (number of sample detected from 24 replicates). DNA amounts are shown in fg.
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Virology Journal

ISSN: 1743-422X