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Table 3 G9R-NA OPXV assay sensitivity to North American OPXV DNA

From: Detection of North American orthopoxviruses by real time-PCR

Virus DNA (fg) 200 20 10 5 2.5 1.25 0.625
Raccoonpox Pure 33 36.5 37.9 38.9 (20) 39.4 (5) NT NT
Skunkpox Pure 28.9 32.7 34 35 36 37.8 (18) 39.2 (11)
Volepox Pure 30.4 33.9 35.2 36.1 37.4 38.8 (23) 39.4 (19)
  1. The average of the cycle when fluorescence crossed the threshold (Ct) for each positive sample is shown.
  2. NT, not tested (number of sample detected from 24 replicates). DNA amounts are shown in fg.